|
|
|
|
For MDs 1. The theoretical basis of infrared spectral blood serum analysis (IRS-BS). 2. Preparation of a patient for IRS-BS. 3. Preparation of blood for IRS-BS. 5. Diagnostic value of IRS-BS. 6. Advantages of IRS-BS over other diagnostic techniques. 7. Medical indications for IRS-BS. 1. The theoretical basis of infrared spectral blood analysis (IRS-BS). Infrared spectroscopy of blood serum (IRS-BS) represents the complex evaluation of biochemical composition of blood serum based on the IR spectra of blood serum. The oscillation range used in IRS-BS contains natural vibrational-rotational frequencies of many molecules (and/or their derivates) of blood serum. The IRS-BS test reveals distinctive traits of specially prepared dried blood serum which are highly specific for different pathological conditions. The diagnostic algorithm is based on the mathematical analysis of distributions on smooth entropy manifolds [1,2]. 2. Preparation of a patient for IRS-BS. A 10 ml vein blood sample should be withdrawn in the morning. 1. The must be no food, medicines (except vital ones) and alcohol (including alcohol-based medicines for internal use) intake at least 8, 24 and 48 hrs, respectively, before venous blood withdrawal. 2. It is not recommended to take IRS-BS test for women with actual menstruation, pregnancy, lactation period. 3. If patient takes a course of medicines or/and probiotics, it is recommended not to perform IRS-BS test for at least 2 month after course's completion. 4. If patient goes through a course of radiotherapy or/and chemotherapy or undergoes radioisotope examination procedure, it is recommended not to perform IRS-BS test for at least 3 month after course's/procedure's completion. 3. Preparation of blood for IRS-BS. First, serum should be obtained from a blood sample using any standard technique (simple sedimentation or spinning in a centrifuge). Then, serum is transferred into a sterile plastic Petri dish and left (uncovered) in a dark place at ambient temperature until it is dried. This step is followed by preparation of the spirit extraction of dried serum with subsequent likewise drying. Then dried serum and its extracts are milled and pressed with crystalline KBr into tablets. The liquid serum may be kept up to 8 hrs at +40С before drying. The dried serum is suitable for IRS-BS for 1 month; it must be stored (in a covered Petri dish) in a dark place at ambient temperature. Absorption spectra of dried blood serum and its extraction (in the form of aforementioned tablets) are registered at 400–7800 cm-1 using Fourier spectrometer. Obtained spectral pictures are then analyzed employing original algorithms developed by "Medical diagnostics" Ltd. 5. Diagnostic value of IRS-BS. The diagnostic accuracy of IRS-BS, as obtained in double-blind randomized clinical trials in Russian Federation: sensitivity is 81-93% and the diagnostic specificity is 95-100% (the specificity in all cases is measured against benign pathology of the relevant organ) for all pathology conditions listed below; the values vary according to certain types of pathology conditions. Pathology conditions that may be detected by IRS-BS: 5.1. Malignant neoplasm of lung: presence or absence. 5.2. Malignant neoplasm of stomach: presence or absence. 5.3. Malignant neoplasm of large intestine: presence or absence. 5.4. Malignant neoplasm of female reproductive system (pelvic part): presence or absence. 5.5. Malignant neoplasm of female breast: presence or absence and, in the case of presence, differentiation between I,II or III,IV stages. 5.6. Malignant neoplasm of prostate: presence or absence. 5.7. Malignant neoplasm of lymphatic system {without further differentiation}: presence or absence. 5.8. Malignant neoplasm of urinary bladder: presence or absence. 5.9. Melanoma (only for women more that 50 y.o.): presence or absence . 5.10. Malignant neoplasm of kidney: presence or absence. 5.11. Benign pathology of female reproductive system (pelvic part) {without further differentiation}: presence or absence. 5.12. Lung tuberculosis (for men only): presence or absence. 5.13. Benign pathology of female breast {without further differentiation}: presence or absence. 5.14 .Benign pathology of lymphatic system {without further differentiation}: presence or absence. 5.15. Benign pathology of stomach {without further differentiation}: presence or absence. 5.16. Benign pathology of large intestine {without further differentiation}: presence or absence. 5.17. Benign pathology of urinary bladder {without further differentiation}: presence or absence. 5.18. Benign pathology of kidney {without further differentiation}: presence or absence. 5.19 . Benign pathology of prostate {without further differentiation}: presence or absence. Benign pathology of lung {without further differentiation}: presence or absence. We recommend to use IRS-BS technique for screening diagnostics of pathological conditions listed in 5.1-5.19 and for differential diagnostics between malignant neoplasms and benign pathology of relevant organs\systems. 6. Advantages of IRS-BS over other diagnostic techniques. The following techniques are commonly used nowadays for oncology screening diagnostics. 6.1. X-ray examination. IRS-BS has the following advantages over X-ray examination: 1) higher sensitivity and specificity at once; 2) ability to detect malignant neoplasms in earlier stages; 3) there is no exposure to X-rays; 4) ability to detect several malignant and benign processes by one blood sample testing. 6.2. Ultrasound examination. IRS-BS has the following advantages over ultrasound examination: 1) higher sensitivity and specificity at once; 2) ability to detect malignant neoplasms in earlier stages; 3) there is no exposure to ultrasound; 4) ability to detect several malignant and benign processes by one blood sample testing. 6.3. Blood test for biochemical serum oncomarkers. IRS-BS demonstrate higher diagnostic accuracy than most of the biochemical serum oncomarkers (regarding malignant neoplasms of organs, that may be examined by IRS-BS test). 7. Medical indications for IRS-BS. We recommend to use IRS-BS for the following purposes. 7.1. Screening cancer diagnostic. 7.2. Screening lung tuberculosis diagnostics. 7.3. Differential diagnostics between cancer and benign pathology. 7.4. Monitoring of cancer treatment . So far, IRS-BS can be done only in the laboratory of "Medical diagnostics" Ltd., Nizhny Novgorod, Russia (see our "Contacts"). All rights on the method of infrared spectral blood serum analysis belong to "Medical diagnostics" Ltd. and are protected by patents of Russian Federation. Postal address: "Medical diagnostics" Ltd., 12A Novikova-Priboya st, Nizhny Novgorod, Russia 603064 Phone: +7-831-2584684. E-mail: md-spectrum@narod.ru |
|
Отправить сообщение для:
md-spectrum@narod.ru с вопросами
и замечаниями об этом веб-узле.
|
